Nylon nanofibrous biosensors for glucose determination
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Nanofibrous membranes have been produced by electrospinning to develop first generation glucose biosensors. The direct immobilization of glucose oxidase onto the polyamide nanofibrous surfaces by drop coating revealed a simple and efficient method for the development of sensitive, stable, and reproducible electrochemical biosensors. The biosensor showed a linear response over the range 1-9 × 10-3 glucose (R2 = 0.9997) with a sensitivity of 1.11 μA/mM and a limit of detection of 2.5 × 10-6 M (S/N = 3). The uncertainty of repeatability was 2% (RSD%, n = 30). After one month of storage, the signal decreased of 35%. The recovery of glucose, evaluated in real samples of honey, was 98% (RSD% = 1%, n = 3).