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dc.contributor.authorMeraviglia V
dc.contributor.authorZanon A
dc.contributor.authorLavdas AA
dc.contributor.authorSchwienbacher C
dc.contributor.authorSilipigni R
dc.contributor.authorDi Segni M
dc.contributor.authorChen HS
dc.contributor.authorPramstaller PP
dc.contributor.authorHicks AA
dc.contributor.authorRossini A
dc.date.accessioned2018-10-29T14:19:08Z
dc.date.available2018-10-29T14:19:08Z
dc.date.issued2015
dc.identifier.issn1940-087X
dc.identifier.urihttp://dx.doi.org/10.3791/52885
dc.identifier.urihttp://hdl.handle.net/10863/6673
dc.description.abstractSomatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by forcing the expression of four transcription factors (Oct-4, Sox-2, Klf-4, and c-Myc), typically expressed by human embryonic stem cells (hESCs). Due to their similarity with hESCs, iPSCs have become an important tool for potential patient-specific regenerative medicine, avoiding ethical issues associated with hESCs. In order to obtain cells suitable for clinical application, transgene-free iPSCs need to be generated to avoid transgene reactivation, altered gene expression and misguided differentiation. Moreover, a highly efficient and inexpensive reprogramming method is necessary to derive sufficient iPSCs for therapeutic purposes. Given this need, an efficient non-integrating episomal plasmid approach is the preferable choice for iPSC derivation. Currently the most common cell type used for reprogramming purposes are fibroblasts, the isolation of which requires tissue biopsy, an invasive surgical procedure for the patient. Therefore, human peripheral blood represents the most accessible and least invasive tissue for iPSC generation. In this study, a cost-effective and viral-free protocol using non-integrating episomal plasmids is reported for the generation of iPSCs from human peripheral blood mononuclear cells (PBMNCs) obtained from frozen buffy coats after whole blood centrifugation and without density gradient separation.en_US
dc.language.isoenen_US
dc.rights
dc.titleGeneration of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmidsen_US
dc.typeArticleen_US
dc.date.updated2018-10-29T14:05:10Z
dc.language.isiEN-GB
dc.journal.titleJournal of visualized experiments
dc.description.fulltextopenen_US


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