Comparative molecular genetic analysis of apple genotypes maintained in germplasm collections
Dalla Via J
MetadataShow full item record
SubjectMalus domestica; Molecular markers; Microsatellite DNA; Cultivar identification; Breeding; Worcester Pearmain; Laxton's Superb
Apple (Malus × domestica) is economically one of the most important fruit crops in the world, with several thousand described varieties of which only a small number is commercially grown. Characterisation of genetic resources of apple is usually based on morphological traits, which are modulated by environmental and ecological factors, and for this reason pomological description needs to be complemented by molecular approaches. Ten accessions assigned to the apple cultivars 'Antonovka', 'Laxton's Superb' and 'Worcester Pearmain' were derived from several germplasm collections in Europe and analysed at 14 variable microsatellite loci. In order to verify their assignment, the molecular genetic data were compared to a database containing molecular genetic profiles of reference varieties. Within the five accessions of different origin maintained as 'Antonovka', two genotypes were identified, which could be assigned as the common 'Antonovka' and 'Antonovka polutorafuntowaja'. All the three accessions of 'Laxton's Superb' displayed the same genotype that was consequently considered to be authentic, and the comparison with the entries of the database enabled to reveal the probable parent pair for this cultivar. For the two accessions of 'Worcester Pearmain', the comparative database approach allowed to recognise a misidentification in one of the two germplasm collections. A comparative analysis of different accessions of a cultivar from independent origins and the constitution of a database are required, in order to contribute to a reliable determination of apple cultivars maintained in germplasm collections. © 2012 The Author(s).
Showing items related by title, author, creator and subject.
Investigating the molecular basis of fire blight by structural and functional genomics of Erwinia amylovora Benini, S; Bartho, JD; Salomone-Stagni, M; Toccafondi, M (2014)The study of the molecular basis of the interaction between Erwinia amylovora and the plant host is of great importance in order to understand the role of the genes involved in determining pathogenicity. Structural and ...
Molecular typing of Staphylococcus aureus isolated from Italian dairy products on the basis of coagulase gene polymorphism, multiple-locus variable-number tandem-repeat and toxin genes Morandi, S; Brasca, M; Lodi, R; Brusetti, L (Cambridge University Press (CUP), 2008)Coagulase gene restriction fragment length polymorphism (RFLP), six-locus variable-number tandem-repeat analysis patterns (MLVA) and detection of enterotoxin genes (se) (sea, sec, sed, seg, seh, sei, sej and sel) were used ...
Zahir A; Pulimeno A; Demarchi D; Graziano M; Piccinini G; Mahmoud A; Lugli P (IEEE, 2015)Recently, several molecular devices have been realized with interesting properties like conductance, switching, gate modulation and negative differential resistance. In this paper we present a new modular framework based ...