Molecular tools applied to the advancement of fruit growing in South Tyrol: A review
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SubjectMolecular typing; Pathogen quantification; Candidatus Phytoplasma mali; Vitis vinifera; Fire-blight; Bois noir; Tilletiopsis spp.; Root grafts; Latent infection; Flavescence dorée; Hyalesthes obsoletus; Malus domestica; White haze; Cacopsylla melanoneura; Grapevine yellows; Molecular diagnostics; Cacopsylla picta; Microsatellite DNA; Cultivar identification; Real-time PCR; Apple proliferation; European stone fruit yellows; Candidatus Phytoplasma prunorum; Scaphoideus titanus; Vector; Erwinia amylovora
Molecular biology techniques have become indispensable tools in various areas of research and routine applications. In South Tyrol (northern Italy), molecular methods have been applied to the field of fruit growing over the last decade, and the main applications have been related to plant pathology and identification of apple cultivars. This review article illustrates how the implementation of existing methods and the development of new assays have contributed to gain more insights about the spread and epidemiology of phytoplasma diseases affecting apple trees and grapevine in South Tyrol, to identify and characterise the causal agents of newly emerging disorders, or to describe the local genetic diversity of the apple. © 2012 The Author(s).
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Molecular typing of 'Candidatus Phytoplasma mali' and epidemic history tracing by a combined T-RFLP/VNTR analysis approach Baric S; Berger J; Cainelli C; Kerschbamer C; Dalla Via J (2011)Apple proliferation caused by 'Candidatus Phytoplasma mali' is a disease of apple trees gaining increasing importance in Europe. The present study describes a high-throughput method for simultaneous typing of 'Ca. P. mali' ...
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