Molecular typing of 'Candidatus Phytoplasma mali' and epidemic history tracing by a combined T-RFLP/VNTR analysis approach
Dalla Via J
MetadataShow full item record
SubjectCacopsylla melanoneura; Cacopsylla picta; Malus domestica; Molecular genetic typing; Rhodanese-like protein gene; Ribosomal protein L22 gene
Apple proliferation caused by 'Candidatus Phytoplasma mali' is a disease of apple trees gaining increasing importance in Europe. The present study describes a high-throughput method for simultaneous typing of 'Ca. P. mali' at two genetic loci. This novel approach combines terminal restriction fragment length polymorphism (T-RFLP) analysis of a putative rhodanese-like protein gene and the analysis of the variable number of tandem repeats (VNTR) of the ribosomal protein L22 gene. The typing approach was applied to analyse a collection of DNA isolates from 310 apple trees tested positive for 'Ca. P. mali'. Samples were taken between 2002 and 2010 in South Tyrol (Northern Italy). In addition, 15 samples of Cacopsylla melanoneura and 19 of C. picta were typed. Seven combined genetic profiles were found in the samples of infected apple trees: AT-2/rpX-A (81.0%), AT-1/rpX-D (8.4%), AT-1/rpX-E (4.2%), AT-1/rpX-A (3.2%), AT-1/rpX-B (1.6%), AT-1/rpX-C (1.0%) and AP/rpX-A (0.3%), and one mixed infection AP + AT-1/rpX-A + rpX-D (0.3%). Subtype rpX-E was discovered for the first time. In C. melanoneura samples the most frequent subtype was AT-1/rpX-E, followed by AT-1/rpX-D and AT-1/rpX-C. All C. picta samples displayed subtype AT-2/rpX-A. Analysis of the temporal distribution of subtype frequencies in apple trees revealed that exclusively subtype AT-1 in combination with four rpX subtypes was present in South Tyrol in the period from 2002 to 2004. From 2006 onwards subtype AT-2/rpX-A became dominant with an average frequency of 90%. The data obtained suggest that there may be a co-adaptation of particular 'Ca. P. mali' subtypes with different insect vector species. © 2011 KNPV.
Showing items related by title, author, creator and subject.
Iakovidou N; Symeonidis P; Manolopoulos Y (IEEE, 2010)An increasing number of observations support the hypothesis that the vast majority of biological functions involve interactions between proteins and that the complexity of living systems arises as a result of such interactions. ...
The non-specific lipid transfer protein N5 of Medicago truncatula is implicated in epidermal stages of rhizobium-host interaction Pii, Y; Molesini, B; Masiero, S; Pandolfini, T (BioMed Central, 2012)BACKGROUND: The symbiotic interaction between leguminous plants and rhizobia involves two processes: bacterial infection, resulting in the penetration of bacteria in epidermal and cortical cells, and root nodule organogenesis. ...
Tomaselli, S; Crescenzi, O; Sanfelice, D; Ab, E; Wechselberger, R; Angeli, S; Scaloni, A; Boelens, R; Tancredi, T; Pelosi, P; Picone, D (American Chemical Society, 2006)Chemical stimuli, generally constituted by small volatile organic molecules, are extremely important for the survival of different insect species. In the course of evolution, insects have developed very sophisticated ...