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dc.contributor.authorFrancavilla R
dc.contributor.authorErcolini D
dc.contributor.authorPiccolo M
dc.contributor.authorVannini L
dc.contributor.authorSiragusa S
dc.contributor.authorDe Filippis F
dc.contributor.authorDe Pasquale I
dc.contributor.authorDi Cagno R
dc.contributor.authorDi Toma M
dc.contributor.authorGozzi G
dc.contributor.authorSerrazanetti D
dc.contributor.authorDe Angelis M
dc.contributor.authorGobbetti M
dc.contributor.editor
dc.date.accessioned2018-05-07T14:41:15Z
dc.date.available2018-05-07T14:41:15Z
dc.date.issued2014
dc.identifier.issn0099-2240
dc.identifier.urihttp://dx.doi.org/10.1128/AEM.00362-14
dc.identifier.urihttp://aem.asm.org/content/80/11/3416
dc.identifier.urihttp://hdl.handle.net/10863/4598
dc.description.abstractThis study aimed to investigate the salivary microbiota and metabolome of 13 children with celiac disease (CD) under a glutenfree diet (treated celiac disease [T-CD]). The same number of healthy children (HC) was used as controls. The salivary microbiota was analyzed by an integrated approach using culture-dependent and -independent methods. Metabolome analysis was carried out by gas chromatography-mass spectrometry-solid-phase microextraction. Compared to HC, the number of some cultivable bacterial groups (e.g., total anaerobes) significantly (P<0.05) differed in the saliva samples of the T-CD children. As shown by community-level catabolic profiles, the highest Shannon's diversity and substrate richness were found in HC. Pyrosequencing data showed the highest richness estimator and diversity index values for HC. Levels of Lachnospiraceae, Gemellaceae, and Streptococcus sanguinis were highest for the T-CD children. Streptococcus thermophilus levels were markedly decreased in T-CD children. The saliva of T-CD children showed the largest amount of Bacteroidetes (e.g., Porphyromonas sp., Porphyromonas endodontalis, and Prevotella nanceiensis), together with the smallest amount of Actinobacteria. T-CD children were also characterized by decreased levels of some Actinomyces species, Atopobium species, and Corynebacterium durum. Rothia mucilaginosa was the only Actinobacteria species found at the highest level in T-CD children. As shown by multivariate statistical analyses, the levels of organic volatile compounds markedly differentiated T-CD children. Some compounds (e.g., ethyl-acetate, nonanal, and 2-hexanone) were found to be associated with T-CD children. Correlations (false discovery rate [FDR],<0.05) were found between the relative abundances of bacteria and some volatile organic compounds (VOCs). The findings of this studyindicated that CD is associated with oral dysbiosis that could affect the oral metabolome. © 2014, American Society for Microbiologyen_US
dc.language.isoenen_US
dc.rights
dc.titleSalivary Microbiota and Metabolome Associated with Celiac Diseaseen_US
dc.typeArticleen_US
dc.date.updated2017-11-04T09:35:44Z
dc.publication.title
dc.language.isiEN-GB
dc.journal.titleApplied and Environmental Microbiology
dc.description.fulltextopenen_US


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