Species diversity and community composition of native arbuscular mycorrhizal fungi in apple roots are affected by site and orchard management
MetadataShow full item record
SubjectClone libraries; Mycorrhizal colonization; PCR-DGGE; Native AMF communities; Small ribosomal subunit (SSU rDNA); Apple trees
Arbuscular mycorrhizal fungi (AMF) are beneficial microrganisms which establish mutualistic symbioses with the roots of most food crops, improving plant performance, nutrient uptake and tolerance to biotic and abiotic stresses. A better understanding of the factors affecting AMF occurrence and diversity is fundamental to implement sustainable agricultural managements effectively profiting from beneficial plant symbionts. Here, we investigated AMF occurrence, diversity and community composition in the roots of apple trees from 21 orchards in South Tyrol, as affected by location, management (organic vs integrated) and altitude, by PCR cloning and sequencing and PCR-DGGE of partial 18S rRNA gene. The screening of 448 clones from 21 clone libraries allowed the identification of 6 native AMF at the species level: Glomus indicum, Sclerocystis sinuosa, Funneliformis mosseae, Rhizoglomus irregulare, Septoglomus constrictus and Claroideoglomus lamellosum. The most abundant genera were represented by Glomus (29.7% of the sequences), Paraglomus (19.4%), Claroideoglomus (17.2%), Sclerocystis (16.1%) and Rhizoglomus (12.3%). Septoglomus, Diversispora and Funneliformis sequences corresponded to less than 4% of total sequences. Although the degree of root colonization was unaffected by treatments, ANOSIM analysis of PCR-DGGE clusters revealed significant differences in apple root AMF diversity between sites and agricultural managements. Species richness was significantly higher in organically managed orchards than in integrated ones. Our findings provide insights into important factors affecting native AMF communities of apple trees, which could be exploited in sustainable fruit production systems, where beneficial soil biota boost biogeochemical cycles, energy fluxes and crop productivity.
Showing items related by title, author, creator and subject.
Assessment of beef production from Brahman x Thai native and Charolais x Thai native crossbred bulls slaughtered at different weights. II: Meat quality Waritthitham, A; Lambertz, C; Langholz, HJ; Wicke, M; Gauly, M (ELSEVIER SCI LTDElsevier, 2010)The objective was to assess meat quality of Brahman x Thai native (BRA) and Charolais x Thai native (CHA) crossbred bulls. In total 34 BRA and 34 CHA under practical farm conditions were randomly assigned for slaughter at ...
Assessment of beef production from Brahman x Thai native and Charolais x Thai native crossbred bulls slaughtered at different weights. I: Growth performance and carcass quality Waritthitham, A; Lambertz, C; Langholz, HJ; Wicke, M; Gauly, M (ELSEVIER SCI LTDElsevier, 2010)Effects of genotype and slaughter weight on growth performance and carcass quality of Brahman x Thai native (BRA) and Charolais x Thai native (CHA) crossbred bulls were studied. Thirty-four BRA and 34 CHA bulls raised under ...
Listening efficiency in university classrooms: A comparison between native and non-native listeners Visentin C; Prodi N; Torresin S; Cappelletti F; Gasparella A (Swiss Acoustical Society, 2017)When listening to speech in one’s native language a higher intelligibility is expected than when listening in a second language: perceptual and linguistic cues readily available for native listeners may be only partly ...