Conservation of Erwinia amylovora pathogenicity-relevant genes among Erwinia genomes
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The Erwinia genus comprises species that are plant pathogens, non-pathogen, epiphytes, and opportunistic human pathogens. Within the genus, Erwinia amylovora ranks among the top 10 plant pathogenic bacteria. It causes the fire blight disease and is a global threat to commercial apple and pear production. We analyzed the presence/absence of the E. amylovora genes reported to be important for pathogenicity towards Rosaceae within various Erwinia strains genomes. This simple bottom-up approach, allowed us to correlate the analyzed genes to pathogenicity, host specificity, and make useful considerations to drive targeted studies.
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Cloning, expression, purification, crystallization and preliminary X-ray analysis of EaLsc, a levansucrase from Erwinia amylovora Caputi, L; Cianci, M; Benini, S (International Union of Crystallography, 2013)The Gram-negative bacterium Erwinia amylovora is a destructive pathogen of Rosaceae. During infection, E. amylovora produces the exopolysaccharide levan, which contributes to the occlusion of plant vessels, causing the ...
Biomolecular characterization of the levansucrase of erwinia amylovora, a promising biocatalyst for the synthesis of fructooligosaccharides Caputi, L; Nepogodiev, SA; Malnoy, M; Rejzek, M; Field, RA; Benini, S (American Chemical Society, 2013)Erwinia amylovora is a plant pathogen that affects Rosaceae, such as apple and pear. In E. amylovora the fructans, produced by the action of a levansucrase (EaLsc), play a role in virulence and biofilm formation. Fructans ...
Investigating the molecular basis of fire blight by structural and functional genomics of Erwinia amylovora Benini, S; Bartho, JD; Salomone-Stagni, M; Toccafondi, M (2014)The study of the molecular basis of the interaction between Erwinia amylovora and the plant host is of great importance in order to understand the role of the genes involved in determining pathogenicity. Structural and ...