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dc.contributor.authorGiorno F
dc.contributor.authorGuerriero G
dc.contributor.authorBiagetti M
dc.contributor.authorCiccotti AM
dc.contributor.authorBaric S
dc.date.accessioned2019-08-28T13:29:52Z
dc.date.available2019-08-28T13:29:52Z
dc.date.issued2013
dc.identifier.issn0981-9428
dc.identifier.urihttp://dx.doi.org/10.1016/j.plaphy.2013.05.040
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0981942813002155
dc.identifier.urihttps://bia.unibz.it/handle/10863/10351
dc.description.abstract'Candidatus Phytoplasma mali' (Ca. P. mali) is the disease agent causing apple proliferation (AP), which has detrimental effects on production in many apple growing areas of Central and Southern Europe. The present study investigated transcriptional and biochemical changes related to the sugar metabolism as well as expression of pathogenesis-related (PR) protein genes in in vitro micro-propagated AP-infected and healthy control plantlets with the aim of shedding light on host plant response to `Ca. P. mali' infection. Expression changes between infected and control plantlets were detected by quantitative real-time PCR analysis. The most significant transcriptional changes were observed for genes coding for pathogenesis-related proteins and for heat shock protein 70, as well as for some genes related to the sugar metabolism, such as a sorbitol transporter (SOT5), hexokinase, sucrose-phosphate synthase or granule bound starch synthase. Furthermore, biochemical analyses revealed that infected plantlets were characterized by a significant accumulation of starch and sucrose, while hexoses, such as glucose and fructose, and sorbitol were present at lower concentrations. In summary, the present analysis provides an overview of a gene set that is involved in response to phytoplasma infection and, therefore, it may help for a better understanding of the molecular mechanisms involved in phytoplasma-host plant interaction in apple. (c) 2013 Elsevier Masson SAS. All rights reserved.en_US
dc.languageEnglish
dc.language.isoenen_US
dc.publisherELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIERen_US
dc.relation
dc.rights
dc.titleGene expression and biochemical changes of carbohydrate metabolism in in vitro micro-propagated apple plantlets infected by `Candidatus Phytoplasma mali'en_US
dc.typeArticleen_US
dc.date.updated2019-08-27T03:00:56Z
dc.language.isiEN-GB
dc.journal.titlePlant Physiology and Biochemistry
dc.description.fulltextreserveden_US


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