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Real-time precise microfluidic droplets label-sequencing combined in a velocity detection sensor
Journal article   Open access   Peer reviewed

Real-time precise microfluidic droplets label-sequencing combined in a velocity detection sensor

Riccardo Zamboni, A Zaltron, M Chauvet and C Sada
Scientific Reports, Vol.11(1), pp.1-12
11
2021
Handle:
https://hdl.handle.net/10863/51656

Abstract

Droplets microfluidics is broadening the range of Lab on a Chip solutions that, however, still suffer from the lack of an adequate level of integration of optical detection and sensors. In fact, droplets are currently monitored by imaging techniques, mostly limited by a time-consuming data post-processing and big data storage. This work aims to overcome this weakness, presenting a fully integrated opto-microfluidic platform able to detect, label and characterize droplets without the need for imaging techniques. It consists of optical waveguides arranged in a Mach Zehnder’s configuration and a microfluidic circuit both coupled in the same substrate. As a proof of concept, the work demonstrates the performances of this opto-microfluidic platform in performing a complete and simultaneous sequence labelling and identification of each single droplet, in terms of its optical properties, as well as velocity and lengths. Since the sensor is realized in lithium niobate crystals, which is also highly resistant to chemical attack and biocompatible, the future addition of multifunctional stages into the same substrate can be easily envisioned, extending the range of applicability of the final device.
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url
https://www.nature.com/articles/s41598-021-97392-3View

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