Generation of human induced pluripotent stem cell line EURACi015-A from a patient affected by dilated cardiomyopathy carrying the Lamin A/C p.Glu161Lys mutation

Giada Cattelan; Laura Sophie Frommelt; Chiara Volani; A Colliva; G Ciucci; A Paldino; M Dal Ferro; M Di Segni; R Silipigni; Peter P. Pramstaller; Marzia De Bortoli; S Zacchigna; Alessandra Rossini

doi:10.1016/j.scr.2023.103172

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Generation of human induced pluripotent stem cell line EURACi015-A from a patient affected by dilated cardiomyopathy carrying the Lamin A/C p.Glu161Lys mutation

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Generation of human induced pluripotent stem cell line EURACi015-A from a patient affected by dilated cardiomyopathy carrying the Lamin A/C p.Glu161Lys mutation

Giada Cattelan, Laura Sophie Frommelt, Chiara Volani, A Colliva, G Ciucci, A Paldino, M Dal Ferro, M Di Segni, R Silipigni, Peter P. Pramstaller, …

Stem Cell Research, Vol.71

28/07/2023

DOI: https://doi.org/10.1016/j.scr.2023.103172

Handle:

https://hdl.handle.net/10863/36310

Abstract

Dilated cardiomyopathy (DCM) is a common heart disorder caused by genetic and non-genetic etiologies, characterized by left ventricular dilatation and contractile dysfunction. Here, we created a human induced pluripotent stem cell line from peripheral blood mononuclear cells using non-integrating vectors from a patient carrying a heterozygous LMNA variant (c.481G > A, p.Glu161Lys, NM_170707.4). The obtained EURACi015-A line, showed the typical morphology of pluripotent cells, normal karyotype and exhibited pluripotency markers and a trilineage differentiation potential. This cell line can be successfully differentiated into cardiomyocytes and endothelial cells. This line represents a human in vitro model to study the genetic basis of DCM.

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Title: Generation of human induced pluripotent stem cell line EURACi015-A from a patient affected by dilated cardiomyopathy carrying the Lamin A/C p.Glu161Lys mutation
Creators: Giada Cattelan - Institute for Biomedicine
Laura Sophie Frommelt - University of Trieste
Chiara Volani - University of Milan
A Colliva - University of Trieste
G Ciucci - University of Trieste
A Paldino - Azienda Sanitaria Universitaria Integrata di Trieste
M Dal Ferro
M Di Segni - Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
R Silipigni - Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
Peter P. Pramstaller - Institute for Biomedicine
Marzia De Bortoli - Institute for Biomedicine
S Zacchigna - University of Trieste
Alessandra Rossini - Institute for Biomedicine
Publication Details: Stem Cell Research, Vol.71
ISSN: 1873-5061
EISSN: 1876-7753
Series / Volume: 71
Publisher: Elsevier
Number of pages: 5
Identifiers: (EURAC)27215433
991006608198701241
Web of Science ID: WOS:001052539000001
Academic Unit: Institute for Biomedicine
Language: English
Resource Type: Journal article
Description coverage: international
Description audience: Scientific
Local Fields: Scientific
Author Names String: Cattelan G, Frommelt LS, Volani C, Colliva A, Ciucci G, Paldino A, Dal Ferro M, Di Segni M, Silipigni R, Pramstaller PP, De Bortoli M, Zacchigna S, Rossini A.

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