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The Use of Spiroplasma melliferum as a Model Organism to Study Potential Antimicrobials Against Phytoplasma
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The Use of Spiroplasma melliferum as a Model Organism to Study Potential Antimicrobials Against Phytoplasma

Amir Fine, Luca Galtarossa and Katrin Janik
Phytoplasma - Methods and Protocols, Vol.3008, pp.209-218
Methods in Molecular Biology, Springer Nature
2026
DOI: https://doi.org/10.1007/978-1-0716-5104-9_16
Handle:
https://hdl.handle.net/10863/53666
PMID: 41528710

Abstract

Anti-Bacterial Agents - pharmacology Anti-Infective Agents - pharmacology Microbial Sensitivity Tests - methods Oxazines - chemistry Phytoplasma - drug effects Phytoplasma - growth & development Spiroplasma - drug effects Spiroplasma - growth & development Xanthenes - chemistry
Spiroplasma melliferum is a cultivable mollicute that can be used as a proxy model organism to test the inhibitory effects of filtrates, compounds, or substances against phytoplasma. In this protocol, we describe the use of the resazurin-based alamarBlue™ dye for the monitoring of Spiroplasma survival and growth in the presence of presumably inhibitory agents.

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